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omniscript reverse transcriptase kit  (Qiagen)


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    Structured Review

    Qiagen omniscript reverse transcriptase kit
    Omniscript Reverse Transcriptase Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 6786 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/omniscript+reverse+transcriptase/Reverse+transcriptase/pm41928441-371-18-22
    Average 97 stars, based on 6786 article reviews
    omniscript reverse transcriptase kit - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Synthesized:

    Article Title: Exercise training improves mitochondrial oxidative energy metabolism through PGC-1α-dependent transcriptional pathway in the aged rat heart.
    Article Snippet: .. Single-stranded cDNA was synthesized from 2 μg of total RNA using oligo (dT) primers and Omniscript reverse transcriptase (QIAGEN, Tokyo, Japan). .. Real-time quantitative PCR was performed using TaqMan Gene Expression assays (Applied Biosystems, Foster City, CA, USA) for PGC-1α (assay ID, Rn00580241_m1; Applied Biosystems), NRF-1 (assay ID, Rn01455958_m1; Applied Biosystems), TFAM (assay ID, Rn00580051_m1; Applied Biosystems), COX VIc (assay ID, Rn00665001_g1; Applied Biosystems), Cyt c (assay ID, Rn00820639_g1; Applied Biosystems).

    Article Title: Piezo1 in hypertrophic chondrocytes regulates osteoclastogenesis in endochondral ossification
    Article Snippet: siRNA-mediated knockdown of Notch3 was achieved by using the silencer-select Notch3 siRNA (#155632, ThermoFisher) according to a previously published protocol . .. Total RNA was isolated from cultured ATDC5 cells and C28/I2 cells using the RNeasy Mini Kit (Qiagen), and each sample was treated with DNase (Qiagen). cDNA was synthesized using Omniscript Reverse Transcriptase (Qiagen) and semi-quantitative real-time PCR was performed using PlatinumTM SYBRTM Green qPCR SuperMix-UDG (Thermo Fisher Scientific). .. Total RNA was isolated from cultured ATDC5 cells and C28/I2 cells using the RNeasy Mini Kit (Qiagen), and each sample was treated with DNase (Qiagen).

    Reverse Transcription:

    Article Title: Exercise training improves mitochondrial oxidative energy metabolism through PGC-1α-dependent transcriptional pathway in the aged rat heart.
    Article Snippet: .. Single-stranded cDNA was synthesized from 2 μg of total RNA using oligo (dT) primers and Omniscript reverse transcriptase (QIAGEN, Tokyo, Japan). .. Real-time quantitative PCR was performed using TaqMan Gene Expression assays (Applied Biosystems, Foster City, CA, USA) for PGC-1α (assay ID, Rn00580241_m1; Applied Biosystems), NRF-1 (assay ID, Rn01455958_m1; Applied Biosystems), TFAM (assay ID, Rn00580051_m1; Applied Biosystems), COX VIc (assay ID, Rn00665001_g1; Applied Biosystems), Cyt c (assay ID, Rn00820639_g1; Applied Biosystems).

    Article Title: The myeloid SRC family kinase HCK regulates breast cancer growth by activating tumor-associated macrophage-led invasion and inhibiting cytotoxic T cell activity
    Article Snippet: Py8119 cells were grown in Ham’s F-12K media (Thermo Fisher Scientific) with 5% FCS, 0.1% Mito serum extender (Corning, Clayton, Vic), 50 μg/ml gentamicin and 2.5 μg/ml amphotericin B (Thermo Fisher Scientific). .. RNA extraction was carried out using the RNeasy protocol and converted to cDNA using Omniscript reverse transcriptase, according to the manufacturer’s instructions (Qiagen, Clayton, Vic). ..

    Article Title: RNA-based discovery and correction of splicing defects caused by POLR3A missense mutations
    Article Snippet: .. 500–1000 ng of RNA was reverse transcribed using OmniScript reverse transcriptase (Qiagen) with a mixture of oligo(dT) and random hexamers. ..

    Article Title: Intravenous high mobility group box 1 fragment improves cardiac function, fibrosis, and coronary flow in porcine ischemic cardiomyopathy model
    Article Snippet: Infarct borders and remote regions of the extracted heart samples were immersed in RNA (Invitrogen). .. Total RNA was isolated using an RNeasy Kit (Qiagen, Hilden, Germany) and reverse transcribed using Omniscript Reverse Transcriptase (Qiagen). .. Real-time polymerase chain reaction was performed using TaqMan Gene Expression Assay Master Mix (Applied Biosystems, Foster City, Calif) on the 7500 Fast Real-Time PCR System (Applied Biosystems, Foster City, Calif).

    Article Title: Intravenous high mobility group box 1 fragment improves cardiac function, fibrosis, and coronary flow in porcine ischemic cardiomyopathy model.
    Article Snippet: RT-PCR Infarct borders and remote regions of the extracted heart samples were immersed in RNA (Invitrogen). .. Total RNA was isolated using an RNeasy Kit (Qiagen, Hilden, Germany) and reverse transcribed using Omniscript Reverse Transcriptase (Qiagen). .. Realtime polymerase chain reaction was performed using TaqMan Gene Expression Assay Master Mix (Applied Biosystems, Foster City, Calif) on the 7500 Fast Real-Time PCR System (Applied Biosystems, Foster City, Calif).

    Article Title: Piezo1 in hypertrophic chondrocytes regulates osteoclastogenesis in endochondral ossification
    Article Snippet: siRNA-mediated knockdown of Notch3 was achieved by using the silencer-select Notch3 siRNA (#155632, ThermoFisher) according to a previously published protocol . .. Total RNA was isolated from cultured ATDC5 cells and C28/I2 cells using the RNeasy Mini Kit (Qiagen), and each sample was treated with DNase (Qiagen). cDNA was synthesized using Omniscript Reverse Transcriptase (Qiagen) and semi-quantitative real-time PCR was performed using PlatinumTM SYBRTM Green qPCR SuperMix-UDG (Thermo Fisher Scientific). .. Total RNA was isolated from cultured ATDC5 cells and C28/I2 cells using the RNeasy Mini Kit (Qiagen), and each sample was treated with DNase (Qiagen).

    Article Title: Aerobic exercise-induced irisin secretion is associated with improved endothelial function and reduced atherosclerosis in ApoE-deficient mice.
    Article Snippet: .. AR TIC L IN PR ES S ARTICLE IN PRESS abdominal aorta using Isogen reagent (Nippon Gene, Toyama, Japan) and either the RNeasy Mini Kit for the soleus muscle (QIAGEN, Hilden, Germany) or the ReliaPrep RNA Miniprep Systems for aorta samples (Promega Corporation, Madison, WI, USA), and then reverse-transcribed using OmniScript Reverse Transcriptase (QIAGEN) according to previous studies11. .. FNDC5 mRNA expression in the soleus muscle and vascular cell adhesion molecule-1 (VCAM1) and intercellular cell adhesion molecule-1 (ICAM-1) expression in the aorta were quantified by real-time PCR (Prism 7500 Fast Sequence Detection System 2.2, Applied Biosystems, Carlsbad, CA) using TaqMan Gene Expression Assays (FNDC5: Mm01181543_m1; VCAM-1: Mm_01320970_m1; ICAM-1: Mm_00516023_m1; Applied Biosystems, Carlsbad, CA), as previously described11.

    Article Title: Aerobic exercise-induced irisin secretion is associated with improved endothelial function and reduced atherosclerosis in ApoE-deficient mice
    Article Snippet: The percentage of the lesion area relative to the lumen area was determined using ImageJ software (version 1.54 g; NIH, Bethesda, MD, USA). .. Total RNA was extracted from the soleus muscle, thoracic aorta, and abdominal aorta using Isogen reagent (Nippon Gene, Toyama, Japan) and either the RNeasy Mini Kit for the soleus muscle (QIAGEN, Hilden, Germany) or the ReliaPrep RNA Miniprep Systems for aorta samples (Promega Corporation, Madison, WI, USA), and then reverse-transcribed using OmniScript Reverse Transcriptase (QIAGEN, Hilden, Germany) according to previous studies . .. FNDC5 mRNA expression in the soleus muscle and vascular cell adhesion molecule-1 ( VCAM-1 ) and intercellular cell adhesion molecule-1 ( ICAM-1 ) expression in the aorta were quantified by real-time PCR (Prism 7500 Fast Sequence Detection System 2.2, Applied Biosystems, Carlsbad, CA, USA) using TaqMan Gene Expression Assays ( FNDC5 : Mm01181543_m1; VCAM-1 : Mm_01320970_m1; ICAM-1 : Mm_00516023_m1; Applied Biosystems, Carlsbad, CA, USA), as previously described .

    RNA Extraction:

    Article Title: The myeloid SRC family kinase HCK regulates breast cancer growth by activating tumor-associated macrophage-led invasion and inhibiting cytotoxic T cell activity
    Article Snippet: Py8119 cells were grown in Ham’s F-12K media (Thermo Fisher Scientific) with 5% FCS, 0.1% Mito serum extender (Corning, Clayton, Vic), 50 μg/ml gentamicin and 2.5 μg/ml amphotericin B (Thermo Fisher Scientific). .. RNA extraction was carried out using the RNeasy protocol and converted to cDNA using Omniscript reverse transcriptase, according to the manufacturer’s instructions (Qiagen, Clayton, Vic). ..

    Isolation:

    Article Title: Intravenous high mobility group box 1 fragment improves cardiac function, fibrosis, and coronary flow in porcine ischemic cardiomyopathy model
    Article Snippet: Infarct borders and remote regions of the extracted heart samples were immersed in RNA (Invitrogen). .. Total RNA was isolated using an RNeasy Kit (Qiagen, Hilden, Germany) and reverse transcribed using Omniscript Reverse Transcriptase (Qiagen). .. Real-time polymerase chain reaction was performed using TaqMan Gene Expression Assay Master Mix (Applied Biosystems, Foster City, Calif) on the 7500 Fast Real-Time PCR System (Applied Biosystems, Foster City, Calif).

    Article Title: Intravenous high mobility group box 1 fragment improves cardiac function, fibrosis, and coronary flow in porcine ischemic cardiomyopathy model.
    Article Snippet: RT-PCR Infarct borders and remote regions of the extracted heart samples were immersed in RNA (Invitrogen). .. Total RNA was isolated using an RNeasy Kit (Qiagen, Hilden, Germany) and reverse transcribed using Omniscript Reverse Transcriptase (Qiagen). .. Realtime polymerase chain reaction was performed using TaqMan Gene Expression Assay Master Mix (Applied Biosystems, Foster City, Calif) on the 7500 Fast Real-Time PCR System (Applied Biosystems, Foster City, Calif).

    Article Title: Piezo1 in hypertrophic chondrocytes regulates osteoclastogenesis in endochondral ossification
    Article Snippet: siRNA-mediated knockdown of Notch3 was achieved by using the silencer-select Notch3 siRNA (#155632, ThermoFisher) according to a previously published protocol . .. Total RNA was isolated from cultured ATDC5 cells and C28/I2 cells using the RNeasy Mini Kit (Qiagen), and each sample was treated with DNase (Qiagen). cDNA was synthesized using Omniscript Reverse Transcriptase (Qiagen) and semi-quantitative real-time PCR was performed using PlatinumTM SYBRTM Green qPCR SuperMix-UDG (Thermo Fisher Scientific). .. Total RNA was isolated from cultured ATDC5 cells and C28/I2 cells using the RNeasy Mini Kit (Qiagen), and each sample was treated with DNase (Qiagen).

    Cell Culture:

    Article Title: Piezo1 in hypertrophic chondrocytes regulates osteoclastogenesis in endochondral ossification
    Article Snippet: siRNA-mediated knockdown of Notch3 was achieved by using the silencer-select Notch3 siRNA (#155632, ThermoFisher) according to a previously published protocol . .. Total RNA was isolated from cultured ATDC5 cells and C28/I2 cells using the RNeasy Mini Kit (Qiagen), and each sample was treated with DNase (Qiagen). cDNA was synthesized using Omniscript Reverse Transcriptase (Qiagen) and semi-quantitative real-time PCR was performed using PlatinumTM SYBRTM Green qPCR SuperMix-UDG (Thermo Fisher Scientific). .. Total RNA was isolated from cultured ATDC5 cells and C28/I2 cells using the RNeasy Mini Kit (Qiagen), and each sample was treated with DNase (Qiagen).

    Real-time Polymerase Chain Reaction:

    Article Title: Piezo1 in hypertrophic chondrocytes regulates osteoclastogenesis in endochondral ossification
    Article Snippet: siRNA-mediated knockdown of Notch3 was achieved by using the silencer-select Notch3 siRNA (#155632, ThermoFisher) according to a previously published protocol . .. Total RNA was isolated from cultured ATDC5 cells and C28/I2 cells using the RNeasy Mini Kit (Qiagen), and each sample was treated with DNase (Qiagen). cDNA was synthesized using Omniscript Reverse Transcriptase (Qiagen) and semi-quantitative real-time PCR was performed using PlatinumTM SYBRTM Green qPCR SuperMix-UDG (Thermo Fisher Scientific). .. Total RNA was isolated from cultured ATDC5 cells and C28/I2 cells using the RNeasy Mini Kit (Qiagen), and each sample was treated with DNase (Qiagen).



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